For example, we may use these cookies to remember your language preferences. We use these cookies to remember your settings and preferences. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies. You can set your browser to block or alert you about these cookies, but some parts of our services will not work without them. They are usually only set in response to actions made by you which amount to a request for services, such as logging in, using a shopping cart or filling in forms. We use these cookies to ensure our site functions securely and properly they are necessary for our services to function and cannot be switched off in our systems. Corrected the final concentration value for Long PCR Control Primer Pair in Table 4. The following change was made to the 5/17 revision of this document:ġ. In addition to Taq DNA polymerase, the GoTaq® Long PCR Master Mix contains a small amount of DNA polymerase with 3´->5´ exonuclease (i.e., proofreading) activity. The polymerase activity is restored after an initial denaturation cycle at high temperature. This allows reactions to be set up at room temperature. The recombinant Taq DNA polymerase is bound by proprietary antibodies that inhibit activity at lower temperatures. The GoTaq® Long PCR Master Mix uses a blend of hot-start, recombinant Taq DNA polymerase and a recombinant proofreading DNA polymerase. The GoTaq® Long PCR Master Mix requires little or no reagent optimization and can amplify up to 30kb of genomic DNA and 40kb of lower-complexity targets such as plasmid and lambda DNA. Only the template and primers need to be added to amplify your target. The master mix contains everything needed to perform long PCR. GoTaq® Long PCR Master Mix is a ready-to-use solution for long PCR targets.